Coding

Part:BBa_K2443045:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-06)


Gln RS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for use in Escherichia coli.



Source

uniprot.org

References

1) Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M., Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. ACS Synth. Biol. 2: 43-52

2) Yamao F., Inokuchi H., Cheung A., Ozeki H., Soell D. (1982) Escherichia coli glutaminyl-tRNA sythetase. I. Isolation and DNA sequence of the glnS gene. J Biol. Chem 10:11639-11643